2D and 3D Cell Culture
Oxygen and pH are key analytes in the wide field of life sciences, for example biotechnology, cell biology or toxicology. PyroScience offers outstanding solutions for oxygen and pH sensing in a variety of applications such as 2D and 3D cell culture, tissue engineering and Organ-on-a-Chip applications.
Applications of optical sensors have been realized for:
- Detection of dissolved oxygen levels and cellular oxygen demand in 2D and 3D (microfluidic) cell culture systems
- Hypoxia, cancer and stem cell research
- Indirect monitoring of cell viability in toxicological screening studies
- Metabolic evaluation of complex 3D tissue-engineered constructs
- Understanding available oxygen levels inside organ-on-a-chip systems
- Measurements of oxygen gradients in spheroids
- Evaluation of chip material gas permeability for material selection during microfluidic chip fabrication
Contactless oxygen sensors from PyroScience feature non-invasive measurements with optical read-out from the outside, thereby reducing the risk of contamination or leakage. They comprise sensor spots for oxygen, pH and temperature (also available as self-adhesive and sterilized version) and oxygen nanoprobes for dissolution in aqueous liquids like culture media. Fixed fiber-based microsensors can be used for profiling in spheroids and for measurements of oxygen gradients at high spatial resolution. All oxygen sensors from PyroScience can be read-out with our multi-channel PC-operated FireSting®-O2, multi-analyte meter FireSting®-PRO (also in combination with our optical pH sensors), or stand-alone with our pocket oxygen meter FireSting®-GO2.
Handouts
Applicable Sensor Types and Products
- Optical oxygen, pH, temperature sensor spots (OXSP5, PHSP5-PK7, TPSP5) or self-adhesive sterilized sensor spots (OXSP5-ADH-STER, PHSP5-PK7-ADH-STER, TPSP5-ADH-STER) with accessories for contactless read-out
- Water-dispersible oxygen sensitive nanoparticles (OXNANO) with accessories for contactless read-out
- Fixed fiber oxygen microsensors (OXF50) with microprofiling unit MM33 or MU1 with stand
Related Peer-Reviewed Publications
High-throughput organ-on-chip platform with integrated programmable fluid flow and real-time sensing for complex tissue models in drug development workflows
Azizgolshani et al. 2021, Lab Chip
https://doi.org/10.1039/D1LC00067E
The material-enabled oxygen control in thiol-ene microfluidic channels and its feasibility for subcellular drug metabolism assays under hypoxia in vitro
Kiiski et al. 2021, Lab on a Chip
https://doi.org/10.1039/D0LC01292K
In vitro assay forsingle-cell characterization of impaired deformability in red blood cells under recurrent episodes of hypoxia
Qiang et al. 2021, Lab on a Chip
https://doi.org/10.1039/D1LC00598G
Fate-mapping post-hypoxic tumor cells reveals a ROS-resistant phenotype that promotes metastasis
Godet et al. 2020, Nature Communications
https://doi.org/10.1038/s41467-019-12412-1
Fluorinated Chitosan Microgels to Overcome Internal Oxygen Transport Deficiencies in Microtissue Culture Systems
Patil et al. 2020, Advanced BioSystems
https://doi.org/10.1002/adbi.201900250