Home  - About Us  - Legal Imprint
applications
 

Effects of temperature fluctuations on the metabolic rate and aerobic capacity of fish

undefined

University of Glasgow, Scotland (UK)

In our lab we are examining the effects of acute temperature fluctuations on the metabolic rate and aerobic capacity of various marine and freshwater fish species. As part of this work, fish are subjected to acute temperature fluctuations similar to what they would experience on a daily basis in the wild. FireSting oxygen sensors and meters are ideal for these kinds of experiments because they offer temperature compensation for changes in water oxygen saturation. This allows us to obtain real-time measures of animal oxygen consumption in respirometry chambers during shifts in temperature.

Fig. 1: A 4-channel oxygen meter FireSting O2 connected to a PC with cables running to oxygen and temperature sensors in an experimental tank.

Fig. 2: Close-up view of respirometry chambers in experimental tank with minnows inside. Robust oxygen miniprobes OXROB3 are inserted into the chambers through sealable valves and can measure the drop in water oxygen concentration as oxygen is used by the fish. The rate of oxygen decrease can be used to estimate the animal’s metabolic rate.

Fig. 3: Example of dissolved oxygen concentration versus time for three respirometers, each containing one European minnow. During the measurement phase, the respirometry chambers are sealed and the rate of oxygen decrease can be used as an estimate of individual metabolic rate. During the flushing phase, aerated water is moved through the chambers to replenish water oxygen supply.